FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905876
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12200.040.1309248
GTCTCGC13650.037.4542121
CTGTCGC14100.036.255129
TCGCTCT15250.029.7451234
GTCTTGC28450.028.3472351
TCTCGCT18100.028.2443962
GTATTAG9300.027.873691
CTCGCTC17300.026.6370683
GTGTTAG23100.025.872661
GTCTAAG10200.025.4142481
GGTATAC5500.024.8751581
GGGTTTC51550.024.0256251
GTTTCAC47350.023.4181863
GTACTAG4650.023.2280751
GTTAGCC17050.023.2268773
GTCTCAC35050.023.0093821
GTATTCG2202.3515062E-522.9113311
GTATTAT8850.022.7818871
ACCGTGT9150.022.0326678
GTGTTAT11650.021.6330171
Produced by FastQC (version 0.11.5)