FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905876
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.045.0420658
GTCTCGC14900.039.1970751
CTGTCGC14650.035.876669
TCGCTCT15450.034.484984
GTCTTGC26150.031.4331651
GATCGTT3151.8189894E-1229.7138969
TCTCGCT18100.029.4431692
CGCTCTG17400.027.7237745
CTCGCTC18100.027.0493683
GGATCGT3204.5474735E-1126.9996458
GGTATAC5750.025.0793971
AGTACCG1801.5945114E-423.9996855
GTGTAAG14550.023.291091
GTCTTAC11600.022.3768771
GTATAAA12450.022.0074231
GTACTAG6250.021.9193941
GTTTCAC47650.021.153973
GGGTTTC52550.021.1301691
GTATAGA8600.020.960251
GTATTGG9350.020.8212641
Produced by FastQC (version 0.11.5)