FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943714
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7650.046.117258
CTGTCGC9950.036.180599
GTCTCGC8250.035.7842751
GTATAGA5500.034.0386961
GTCTTGC18850.033.2330471
TCGCTCT8950.032.9846534
TCTCGCT10150.029.794382
GTATTAG7200.028.001921
GTATTAT6200.027.8728791
CTCGCTC10850.027.208543
GGTATAC3750.026.8818441
CGCTCTG10450.026.8719675
GTATTAA6300.026.287521
GTCTTAC8150.025.621391
GAACCGT2001.1245489E-525.201086
TTGCTCT27850.024.0441194
TAGCCCG1801.5936409E-424.0010285
TAACACG5100.024.0010284
GGATCGT2401.6987815E-623.9997948
GTCTCAC24850.023.470421
Produced by FastQC (version 0.11.5)