FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916148
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC25600.035.159051
TCTGTCG11400.032.84198
TCGCTCT14100.031.1498134
GTCTCGC12300.030.4414441
GTATTAG9400.029.1086981
CTGTCGC13850.028.0720279
CGCTCTG14900.026.0946945
GTCTAGT9950.026.0523221
TCTCGCT15750.025.1439972
GTATTAC5200.023.5403331
TTGCTCT34550.023.3407424
GTGTTAG17300.023.3082121
GTGTAAT10050.022.9271971
GTATAAT7700.022.4433421
GTATAGG6100.022.4280131
CTCGCTC16900.022.1548483
GGAGTGC65550.021.6390535
GTCTACG2003.2519453E-421.6017191
GTATACA11000.021.6017171
ATTAGAC8000.021.6009773
Produced by FastQC (version 0.11.5)