FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693294
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT14550.034.1453934
GTCTCGC12650.033.015341
GTCTTGC24800.030.1967321
TCTGTCG9900.029.8180078
CGCTCTG15500.029.2654325
GTATTAG9700.028.2091621
CTGTCGC11550.028.0517839
TCGCGCA1306.753848E-427.6921449
CTCGCTC16650.027.6770533
TCTCGCT15850.027.2568762
GATCGTT3750.026.879849
GTATTAA7750.025.0865171
ACCGTGT8750.024.6855668
GTTTCAC44450.024.1361833
TTCACCG9400.023.7454225
GGGTTTC50200.023.5243931
GTGTAAA16950.023.3652971
GGTATAC5550.023.3538131
GGTTACG1550.001882444523.2282561
GGATCGT4403.6379788E-1222.9089558
Produced by FastQC (version 0.11.5)