FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693294
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11200.039.8566558
GTCTCGC12600.036.62811
TCGCTCT13350.035.595074
CTGTCGC13600.035.4701549
GTCTTGC26350.030.1035021
CGCTCTG14950.027.9327685
TACACCG2354.6027708E-827.5741315
CTCGCTC15200.027.4733453
GAATACG2456.791561E-826.4899671
TCTCGCT16200.026.2272552
GTATTAC7150.024.2052821
GTTAGCC15800.023.2402213
GTGTTAG18950.022.832161
GTCTTAC10550.022.5562271
GTATAGA9650.022.4181061
GTGTTAA11000.022.2890321
GTATTAT9100.022.1881751
GTATTAG8900.021.8765451
TTATACT9600.021.7497334
TTACACT15200.021.3155274
Produced by FastQC (version 0.11.5)