FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792748
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.042.84818
GTCTCGC12750.037.8896451
CTGTCGC12900.036.2784929
GTCTTGC26800.034.4374541
TCGCTCT14750.033.1932874
GTATTAT8250.030.5893291
GTGTTAC13050.029.8358961
GTACGCC1453.6614823E-529.7931633
GTACGTC1753.9868683E-628.8000563
CGCTCTG16450.026.6991425
GTGTACG3950.025.5556431
TCTCGCT16000.025.2032092
CTCGCTC16250.024.8123553
GTCTTAC11150.023.2800271
TTGCTCT39000.023.2615874
GTGTAAT11700.022.8019371
GTATTGG11150.022.633361
GTGTAAA18350.022.0043131
GGGTTTC50150.021.9976521
GTGTTAT12850.021.8835281
Produced by FastQC (version 0.11.5)