FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2669647
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.041.9151048
GTCTCGC12250.037.6195031
CTGTCGC12800.035.4371729
TCGCTCT14800.032.1090134
CTCGCTC15050.029.1835483
GTCTTGC27200.027.5317361
GTGTTAT10550.027.300881
TCTCGCT15350.027.2059782
CGCTCTG16400.025.903175
TAGACCG1959.243062E-625.8468845
GTATTAC6850.024.1772231
GTATTAT8350.024.145751
TAATACT9950.023.8800724
GTCTCAC30100.023.683061
CCGTGTT8650.023.3061459
GTATTAA9000.022.401891
GTGTTAG18400.022.306231
GTGTAGA14900.022.2300661
GGGTTTC48650.022.2012561
GTCTTAC11400.022.107131
Produced by FastQC (version 0.11.5)