FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2669647
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12300.033.365428
CTGTCGC14250.031.8311639
GTCTCGC11850.031.0299451
GTCTTGC26100.030.6627881
TCGCTCT12350.029.7324094
GTATTAC6100.027.1848641
CGCTCTG13650.026.900755
GTGTACG2955.16593E-1026.8843691
GTACGGA2551.00226316E-725.4467031
GGGTTTC48350.024.3063811
GTTTCAC44700.023.6775443
GTCTCAC29350.023.336981
GTGTTAC10700.022.9099581
TCTCGCT15650.022.5487522
ACTGTCG2903.5176708E-722.3445368
TATACTG8850.021.9658165
CTCGATC11500.021.9127588
GTCTTAC11200.021.8871941
CTCGCTC15800.021.4174423
ACCATGT36150.020.5142578
Produced by FastQC (version 0.11.5)