FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828744
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11350.032.9863978
GTCTCGC13450.032.161221
CTGTCGC14550.029.6903729
TCGCTCT14400.029.4996514
GTCTTGC27400.028.680021
GTACTAG5700.026.5612161
GTGTTAC10900.025.7953621
GGTATAC6750.025.633681
GTACCGC1801.5802002E-424.0315741
TCTCGCT17400.024.0035362
GGTCCGA3102.570232E-823.2563631
CTCGCTC17300.022.8899043
GAGTGCA68900.022.885076
GTCTTAC12150.022.5481451
CGCTCTG16550.022.187055
GTATTAT8800.022.1199721
TAACACG5550.022.0537934
GGAGTGC63900.021.7462225
GTCTCAC32950.021.6612381
GGGTTTC52350.021.6215321
Produced by FastQC (version 0.11.5)