FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210520
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.043.588978
GTCTTGC21900.034.197021
CTGTCGC12050.033.4604159
GTATTAT6650.029.2375661
TCGCTCT12100.027.96684
GTCTCGC10300.027.9654521
GTGTAAC6800.026.4746481
GTGTTAC9550.024.8833961
GTCTACG2051.3606961E-524.5891341
TTGCTCT30500.024.0785664
GTACGGT1500.001554921624.003681
GTATTAA6950.023.8309921
TTACGCT2202.3530449E-522.9089764
GTCTTAC9450.022.8606471
GTGTTAG13950.022.7131581
TACACTG13100.022.5342375
CTTGCTC30800.022.2086873
CGCTCTG12700.022.1101265
GTGTAAG11400.022.1086541
GTATTAG6200.022.06791
Produced by FastQC (version 0.11.5)