FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440696
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10950.045.369648
GTCTCGC11350.045.0403561
CTGTCGC12550.043.0276839
TCGCTCT13100.039.0235144
CTCGCTC13350.036.1353953
CGCTCTG14250.033.853165
TCTCGCT14400.032.500512
GTATTAG7800.028.6158331
GTCTTGC22400.028.6075921
GTATAGG6250.026.4964161
GTCTCAC29350.025.2678591
GTTAGCC11250.024.3203813
TACACTG15100.023.8414335
GTCTTAC9700.023.7529491
GTGTAAA13300.022.7371981
GTACATA7300.022.6852871
GTTTCAC33650.022.680893
GTGTTAG15650.022.5434841
GTATAAG7450.022.2285371
ACCGTGT6850.022.0728868
Produced by FastQC (version 0.11.5)