FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341934
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.045.7332888
GTCTCGC11200.044.4191361
CTGTCGC13300.038.9769179
TCGCTCT12800.038.8119774
TCTCGCT14500.034.2659952
GTCTTGC22700.033.985761
CTCGCTC15750.031.542433
GTATTAA5300.031.2889521
GTATTAG6700.031.2077331
CGCTCTG15250.030.6881125
GTATTAT7100.027.4185471
TTGCTCT29200.025.8900624
GTATAAC5150.025.2002181
GTGTAAA14200.024.8797931
GTGTTAC9450.024.4150281
GTGTACG3301.893568E-924.0335431
GTTTCAC37450.024.031723
GTCTCAC28650.023.9077111
CTTGCTC31300.023.4629443
GTCTTAA7850.022.961981
Produced by FastQC (version 0.11.5)