FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3357688
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14250.037.882691
TCTGTCG13400.035.9842728
TCGCTCT15850.034.0545124
TCTCGCT17250.030.4553452
GTCTTGC27700.029.622351
CTCGCTC17100.029.4608083
GTATTAG10200.028.9319761
GCGTAAT1804.9767896E-627.99111
GGGTTTC48150.027.6547581
CGCTCTG19000.027.6510725
GGTATAC7150.027.1801681
CCGTGTT11150.026.4637669
TATACGG1658.8566296E-526.1703785
GTTCTAC9400.026.0342751
GTGTTAG21650.025.931711
CTGTCGC20100.025.4217229
TTCACCG9550.024.8687115
GTATATA10450.024.7959421
GTTTCAC45300.024.7838693
GTCTTAC12600.024.5636181
Produced by FastQC (version 0.11.5)