FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454054
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10950.046.741931
TCTGTCG9350.046.200248
TCGCTCT11600.039.7215844
TCTCGCT12800.038.2732732
CTGTCGC12650.035.8554049
CTCGCTC12650.033.596673
GTGTTAC9050.032.658611
GTCTTGC19500.030.3138891
CGCTCTG14850.029.573865
TATACTG6400.028.1231925
ACCGTGT7450.028.0250478
GTATAGT6050.027.405321
GTATTAA6400.027.0329651
GCGTTAT1358.3598157E-426.6992231
GTATAAT5500.026.2137831
GTGTTAG15950.025.7618221
GTCTTAC8400.025.7456821
GGAGTGC53300.025.3942415
TATAATA5500.024.8878632
GAGTGCA58200.024.8643826
Produced by FastQC (version 0.11.5)