FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2454054
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9250.039.6841961
TCTGTCG9350.037.7170948
TCGCTCT11900.032.054034
GTCTTGC21250.030.1453421
GTATTAG6450.029.013681
GTATAAT6250.028.7904971
GTGTTAG15600.027.6831681
CTCGCTC12500.027.6366253
GTTAGCC12800.027.551163
TCTCGCT13100.027.467932
GTATAAC4100.026.3327711
GTCTTAC7950.026.2554841
CGCTCTG13450.026.219695
TTACACT11100.025.935274
TGTTAGC13700.025.2143552
TATAATA5800.024.8158552
TAATACT5850.024.6052574
ATAATAC7150.024.1578883
TATTACG1500.001560648123.9886592
TCTCGAT10250.023.8730987
Produced by FastQC (version 0.11.5)