FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4429566
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16650.041.511228
TCGCTCT19350.033.8585934
GTCTCGC17900.033.036611
CTGTCGC21600.032.9981779
GTCTTGC37900.031.3963661
GTCTTAC14600.029.142971
TCTCGCT21050.028.407542
CTCGCTC22000.027.5055353
CGCTCTG22300.026.7967245
GTCTCAC44950.026.6325681
GTGTTAC15300.026.395581
GTATTAG14950.025.0839981
GGGTTTC61550.024.722311
TTGCTCT49000.024.390494
TTACACT18200.023.7349534
GTTTCAC58450.023.6635673
ATACCCG2752.1091728E-723.5623344
TTACTCT22350.022.871224
GGTATAC8600.022.6412261
GTCTCGA40050.022.1111376
Produced by FastQC (version 0.11.5)