FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3893004
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14600.047.339588
GTCTCGC15400.042.1435551
TCGCTCT16300.039.7521744
CTGTCGC20900.035.1366129
GTCTTGC32350.032.99111
TCTCGCT19600.031.9859922
CTCGCTC20200.031.0331213
CGCTCTG20750.030.8800435
GTCTTAC13700.030.5292991
GTGTTAG25750.026.604541
GTATTAC8050.025.9783481
GTCTCAC42750.025.3025631
GTGTAAA22400.025.1105371
GTATATA14750.024.4448491
GTATAAT10350.024.3858051
GGGTTTC57450.024.351241
GTTTCAC51250.024.1819953
GTGTTAC13450.024.1267931
TTGCTCT42650.023.632794
GTATTAG11100.023.3877751
Produced by FastQC (version 0.11.5)