FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3893004
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC30750.036.2799761
TCTGTCG17300.033.2788628
GTCTCGC15000.032.628571
TCGCTCT17200.030.1251134
GTATTAT10800.029.3230631
GTATTAC9850.028.4976331
CCGTGTT12100.027.953569
GTGCTAC13000.027.6826131
GTACTAT7300.027.606771
GTTTCAC50350.027.0138783
ACCGTGT12350.026.8049818
GGGTTTC57750.026.795811
GTATTAG11600.026.680311
TTCACCG10650.026.353585
TAATACT10050.025.7787044
GTGTTAG23250.025.6942251
CGCTCTG19350.025.2902185
TACCCCG4300.025.1042615
TTACACT17250.025.0314944
GTCTTAC12700.024.9361481
Produced by FastQC (version 0.11.5)