FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648409
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8000.046.7988628
GTCTCGC11500.039.501441
TCGCTCT13250.033.1463664
CTCGCTC14700.031.3615723
TCTCGCT14050.030.7651712
CTGTCGC13250.030.429459
GTCTTAC9550.030.2013821
GTCTTGC22550.030.0574051
GGGTTTC37750.029.2243351
CGCTCTG15750.028.3421695
TTACACT11600.027.3096834
TTACGCT1607.163047E-526.9993464
GTTTCAC35500.026.1755963
TTGCTCT27250.025.892954
CGTACTC2301.1681495E-625.055173
GTATTAG8650.024.17421
CCGTACT1801.5945798E-423.9994164
ACGTACT2101.6423004E-523.9994164
GGCTAGA12750.023.7524991
GTGTTAG18550.023.322631
Produced by FastQC (version 0.11.5)