FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648409
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.039.61398
GTCTCGC11800.036.60011
GTCTTGC18750.035.702181
CCGTGTT7450.034.7751549
GTGTAAT9750.031.7451131
TCGCTCT12700.030.5993984
GTATTAC5450.029.0562291
GTATTAG8400.028.2779331
CTGTCGC16650.028.0945439
TCTCGCT15550.027.3051452
GTGTTAC9250.027.2357521
TAATACT6150.026.9138324
GTGTTAG16600.026.884171
GGTATAC6300.026.2784881
CTCGATC9900.026.1691848
TTCACCG7750.026.0003495
CTCGCTC14550.025.719543
GTCTCAC27150.025.1864411
TTACACT11450.025.14074
TCTCGAT10350.025.0313937
Produced by FastQC (version 0.11.5)