FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325844
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12300.046.2414448
GGTATAC6450.039.1253361
GTCTTGC27350.034.5353361
CTGTCGC17300.032.876869
GTCTCGC12650.029.6389311
GTATTAG12850.027.49431
TCGCTCT14200.026.3647944
TTGCTCT36100.026.1260324
TATACTG10400.024.9217515
TTACACT14250.024.7565774
GTCTTAC12550.024.7044091
GTCTCAC34550.024.625421
GTATTAC7050.024.5454981
GTCTCGA28850.023.4580236
TCTCGCT15850.023.1833862
CTTGCTC37300.022.9848353
GTTTCAC45150.022.8182093
GGGTTTC50350.022.7691781
GTGCTAC10500.022.6607531
GTACTAT7150.022.1853541
Produced by FastQC (version 0.11.5)