FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325844
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12000.040.1817448
TCGCTCT14500.031.2685534
GTCTTGC25750.030.4693241
GTCTCGC13750.030.3625931
CTGTCGC17900.029.7518469
GTGTTAC11600.027.9233881
TAATACT10250.027.3826794
GTATTAT10150.026.9482861
GGGTTTC52250.026.8634551
GTTTCAC46250.026.4528473
CTCGCTC15800.025.9628853
TACCCCG3659.094947E-1225.6321895
GTTCTAC8450.025.555131
TTATACT10100.024.9391634
GTTACCG2351.4203415E-624.4995063
GTGTAAA19800.024.3569661
GGTTTCA49850.024.2537752
CGTTTCG1801.5993562E-423.9891019
GTCTTAC12150.023.6972141
TTGCTCT35500.023.313354
Produced by FastQC (version 0.11.5)