FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272524
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12900.050.2288978
GTCTCGC12850.047.703891
TCGCTCT13650.042.1947254
TCTCGCT15500.039.5160142
CTGTCGC17250.037.5624779
CTCGCTC16600.034.720143
GTCTTGC29000.032.5770151
GTATTAT9600.031.5512121
CGCTCTG16700.029.7463365
TAATACT8300.029.4918254
GTATTAG9950.028.2669871
TTGCTCT35450.027.6199914
GTCTTAC13050.027.0784361
GTACTAG7500.026.9237021
GGGTTTC50200.025.8587061
GTCTCAC32950.025.3887081
GTTTCAC44800.025.2476633
GTATTAC6700.024.7566031
GTGTTAC11950.024.7430921
GTATAAT8500.024.6046451
Produced by FastQC (version 0.11.5)