FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272524
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13950.035.0871161
TATACCG1501.2023156E-633.58465
TCTGTCG11350.032.9716648
TCGCTCT13450.029.9639534
GTCTTGC26900.029.4342781
GTATTAC7350.029.3796711
GGTATAC5400.029.3252641
GTTTCAC44650.027.5618293
GTATTAT9500.027.2767051
GTGTTAG22550.026.813021
GGGTTTC52800.026.5835951
CTCGATC12650.026.1698178
CTCGCTC15700.025.6697583
TCTCGCT16700.024.5635872
GGACCGT4151.8189894E-1224.2780236
GTGTTAC12000.023.9933991
ACCGTGT10550.023.8753078
CCGTGTT10250.023.8719799
GTCTCGA27650.023.685346
TCTCGAT13450.023.5431067
Produced by FastQC (version 0.11.5)