FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307520
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13050.051.8513228
GTCTCGC15700.049.3141141
TCGCTCT14550.042.0658574
TCTCGCT17350.038.1915472
GTCTTGC26900.036.5825271
CTGTCGC19600.036.360369
CTCGCTC17750.036.1042983
CGCTCTG17350.034.4377065
GGGTTTC48050.029.967241
TTGCTCT38150.029.8219744
GTATTAG9250.029.7254831
GTTTCAC46950.028.8329773
GTATAAT8450.028.258191
GTCTTAC11350.028.0507141
GTATTAA8550.026.2350981
GGTTTCA47450.026.1078512
GTACTAC5150.025.2902051
TAATACT8450.024.712414
TCGATCT13150.024.6340489
TAACGCA2351.4135603E-624.512974
Produced by FastQC (version 0.11.5)