FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307520
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14600.042.799591
TCTGTCG15250.040.1374938
GTCTTGC28200.037.875831
TCGCTCT17200.035.5870254
CTCGCTC19450.031.8284783
GTATTAA8750.030.72471
GGGTTTC45300.029.9942071
GTTTCAC39750.029.880223
TTGCTCT35700.029.8534624
CGCTCTG18900.029.7189855
TTCACCG8000.029.7046975
TCTCGCT19800.029.4739152
ATGGCGT13550.028.1668176
GTCTTAC12350.027.6518941
TAATACT8300.026.8958194
CCGTGTT9900.026.1859599
GTGTTAC11700.026.082991
CTTGCTC36850.025.980723
TCTTGCT40750.025.8132842
GGTTTCA42700.025.6468322
Produced by FastQC (version 0.11.5)