FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4529359
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18100.047.3272488
GTCTCGC20200.038.693851
GTCTTGC39000.038.0416031
TCGCTCT20100.037.9740524
CTGTCGC26750.031.7555949
TCTCGCT23550.030.8882922
TTGCTCT49350.029.9118864
CGCTCTG23750.029.401225
CTCGCTC24050.029.0412043
GTCTTAC14600.028.7504541
GGGTTTC62550.028.4627841
TTACACT18550.028.3371434
GTTTCAC56700.027.8111533
GTATTAC8900.026.8345011
CTTGCTC47100.026.7531593
GTATTAA10600.026.6273751
GTGTAAC12500.026.053861
GTATTAG12750.025.5430031
GTGTAAA24950.025.5259381
GTATAAT11350.024.8678551
Produced by FastQC (version 0.11.5)