FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829184
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT12200.041.315314
GTCTCGC12650.039.63941
GTCTTGC23400.039.441771
TCTGTCG13200.033.821328
GTTAGCC12600.033.7072833
CGCTCTG14900.032.862115
CTCGCTC15750.032.45043
GTATAGT7000.032.183421
GTCTTAC10350.030.192331
TCTGTCA29450.029.8295948
GTATTAG7750.029.0688951
TCTCGCT16650.028.9925792
GTGTTAA10400.028.6496321
TAGTACT6100.028.3304984
TTGCTCT29700.027.1540364
TGTTAGC14800.026.7748622
GGGTTTC40300.026.5082361
CTTGCTC28600.026.4280763
GTATTCG2507.6632205E-826.1620031
GTGTTAT9200.026.0672151
Produced by FastQC (version 0.11.5)