FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624313
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14850.044.341861
TCTGTCG14000.042.161318
TCGCTCT15700.034.3985144
GTCTTGC30350.034.332291
CTCGCTC17450.033.0116043
CTGTCGC20350.032.5439039
TCTCGCT18850.031.3320262
GTATTAT8550.030.4673941
CGCTCTG18100.029.8270955
GGTATAC6750.028.9440271
TTGCTCT39200.028.8397624
GTACTAC4900.026.581251
GTCTCAC37850.026.5734441
GTATAAG9550.025.7617021
GGGTTTC52550.025.3363531
GTATAAC6000.025.3260251
GTTTCAC46700.024.9787223
GTCTTAC14100.024.6332151
CTTGCTC39650.023.971923
GTATAAT7850.023.9663931
Produced by FastQC (version 0.11.5)