FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094357
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12900.050.7811628
GTCTCGC12750.043.141031
GTCTTGC25400.041.0313531
CTGTCGC18350.040.408549
TCGCTCT13900.038.3311734
TCTCGCT15200.035.0619162
GGTATAC6550.034.2536961
GTTTCAC36800.033.0648883
GGGTTTC45800.032.8688851
GTCTCAC31550.032.5743751
CTCGCTC16150.032.5445673
TTCACCG9600.031.4951065
TTGCTCT30200.031.4703944
ACCGTGT9550.030.1514058
TCACCGT10500.028.7945926
CGCTCTG16550.028.2735485
CTTGCTC31050.027.5939273
GGTTTCA41250.027.5854852
TCACTCT35150.027.0385744
TCTGTTG29600.026.7517348
Produced by FastQC (version 0.11.5)