FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3094357
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14300.043.20471
TCGCTCT15250.039.1922874
TCTGTCG14250.038.4052938
CTCGCTC16200.035.1034663
CGCTCTG17050.034.632345
GTCTTGC25950.033.6118131
TCTCGCT18250.032.765082
GTCTTAC10000.032.7084961
GGGTTTC43850.030.4997521
GTATTAT8950.029.2366471
GTACTAG5250.029.0742231
GTATAAC5500.029.0742191
GTTTCAC40850.028.0183813
TTGCTCT29400.027.4323544
GTATTAC6150.027.1832141
GTGTAAA17750.027.0267411
TTACACT11850.026.7378644
GGTATAC5700.025.5037041
GGTTTCA44150.025.2928052
GTATTAG10500.024.9207611
Produced by FastQC (version 0.11.5)