FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4595807
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16450.047.2686168
GTCTCGC18200.042.825591
TCGCTCT19750.039.006024
GTCTTGC38300.035.990311
TCTCGCT21100.035.8857232
CTGTCGC22950.034.1947029
CTCGCTC23150.032.076543
CGCTCTG23150.031.4112635
TTGCTCT47650.030.9746194
GTACTAT8700.029.0335271
GTTTCAC59850.028.3077263
GGGTTTC60950.027.7072321
TAATACT11700.027.6911954
GTCTTAC14400.027.0633951
GTATTAT13950.026.3843861
GTGTAAA21850.025.7628651
GTATTAG13500.025.6601091
GGTATAC11000.025.587211
TCTGTCA47650.025.5351738
GTCTCAC45850.025.4992081
Produced by FastQC (version 0.11.5)