FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4595807
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC19200.048.385241
TCTGTCG17700.047.592938
TCGCTCT21150.042.5529374
CTCGCTC22650.038.4633453
TCTCGCT25400.036.283242
GTCTTGC34200.034.954761
CGCTCTG25450.034.7974325
CTGTCGC25400.034.015549
GTATTAA12600.032.578321
GTCTTAC15500.026.9476381
GTGTTAG26200.026.9369961
TAATACG2808.8402885E-925.7141324
TTGCTCT46850.025.664734
GTTTCAC56250.025.5998463
GGGTTTC59450.025.4385191
GTGTAAC12150.024.8941541
GTTAGCC21350.023.9436513
GTATTAT11050.023.4619771
GTATAAT13350.023.1959191
GTATAAC8700.023.1773191
Produced by FastQC (version 0.11.5)