FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714430
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10500.041.3601951
TCTGTCG9100.040.341388
GTCTTGC21900.035.033411
TCGCTCT12000.031.8071444
TCTCGCT12050.031.6821692
GTATTAT7250.029.9504851
GTATTAC5600.029.727641
CTGTCGC14250.029.2988859
GTATTAA5950.027.9789561
GGGTTTC35600.027.854231
CTCGCTC13350.027.5107823
GTTTCAC31600.027.3469033
CGCTCTG14000.026.737535
GTGTTAG15250.026.579011
ACCGTGT7100.026.3594888
GTGTTAC9550.025.7689171
TAACACT10900.025.7672564
GTGTAAC8150.025.7549691
GTTCGGA2001.0800924E-525.3331181
TTCACCG7700.025.2417225
Produced by FastQC (version 0.11.5)