FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787419
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11150.042.6097768
GTCTCGC12050.039.638481
TCGCTCT11800.038.4463044
GTCTTGC21750.035.602841
TCTCGCT13450.034.2720032
CTGTCGC16350.032.141699
GTTTCAC34900.031.7748533
CTCGCTC15200.030.3196813
GGGTTTC40950.030.0438211
TTGCTCT29550.029.4865474
TTCACCG7200.028.9943565
GTGTTAG17000.028.9481051
GTGTAAC7000.028.9481031
GTGTAAA14850.027.2911421
GTATTAT7200.027.1388471
GTCTCAC29700.027.0474721
CGCTCTG15450.027.0238675
GTCTTAC8650.026.7728141
GTATTAA6600.026.316461
GGTTTCA38750.026.2077162
Produced by FastQC (version 0.11.5)