FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2787419
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11100.042.8149958
GTCTTGC22000.038.635771
GTCTCGC12450.036.178351
TCGCTCT13200.035.458014
CGCTCTG14950.033.2340165
GGGTTTC36200.030.102951
GTCTTAC7800.029.8044951
GTTTCAC33650.029.306923
GTACTAA4500.029.0593811
CTCGCTC16100.027.2733573
TCTCGCT16200.026.6798042
TTGCTCT29000.026.0715124
CTTGCTC28750.025.7889733
GTATATA8800.025.592071
GGTTTCA36500.025.4591122
TACGGTT1450.00127677124.8300134
GTCTCAC26650.024.806791
TCTTGCT33400.024.8026332
TAATACT7000.024.6881264
GTGTAAA14600.024.3820131
Produced by FastQC (version 0.11.5)