FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485935
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT10250.040.039554
GTCTCGC10350.039.1500051
TCTGTCG9650.034.3134088
GTATTAT5700.034.2746541
TCTCGCT11350.034.263542
GTCTTGC19900.032.7245451
GTTTCAC31950.031.9997773
GGGTTTC37100.031.5955051
GTATTCG1851.6739068E-731.2897721
CTCGCTC12400.030.1933353
GGTATAC5500.028.943041
CGCTCTG14150.027.9800425
TAATACT6150.026.9271834
TTACACT9600.026.2503434
GGTTTCA35000.026.131842
TTCACCG6650.025.9795535
GTCTATA4550.025.4444331
GTGTTAG15650.025.429191
TTTCACC34550.025.2159634
CTGTCGC15050.024.87249
Produced by FastQC (version 0.11.5)