FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070874
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12450.041.728651
TCTGTCG12500.039.7424438
GTCTTGC24750.039.0661621
TCGCTCT13950.038.1920554
TCTCGCT14700.033.3531532
CTGTCGC16200.032.8876049
GTTTCAC38100.029.703923
CTCGCTC16400.029.4489693
TTGCTCT33300.028.7556324
GTATAAT6850.028.4409681
TAATACG2054.297508E-728.0964624
CGCTCTG16100.027.7256225
TACACCG2604.0145096E-927.6912255
GGGTTTC43900.027.4487841
CTTGCTC32800.026.5919783
GTATTAC6000.026.4571231
GTCTTAC9550.026.4445291
TAGTACT5600.025.7132784
GTGTTAG18800.025.3312871
GTACTAG5950.024.2540471
Produced by FastQC (version 0.11.5)