FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268765
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8900.038.0283281
TCTGTCG8250.035.7818078
CCGATAA850.00283736533.882349
TCGCTCT9150.033.8360564
GTCTTGC17550.032.8255651
GTGTAAC6000.031.2048031
TCTCGCT11800.029.8982942
GTATTAA5900.029.2926451
CTCGCTC11350.028.5462463
TACTGCG2553.259629E-928.2352897
GTCTTAC7350.027.4327961
CGCTCTG11550.027.4285625
GTGTTAG14450.026.9107171
GTATTAT5700.026.53041
TAATACT5000.025.9199924
GCGTAAT1400.001040113625.7182461
TTACTCT11300.025.486724
GTCTAAC4300.025.1201461
CTGTCGC12650.024.4743029
TTGCTCT24350.024.24644
Produced by FastQC (version 0.11.5)