FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107705
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10350.043.1294988
GTCTTGC22100.036.8938141
TCGCTCT11400.036.630784
GTCTCGC12150.035.0383071
GTACTAC3950.032.8808331
GTATTAA6350.030.6801451
CTGTCGC15550.030.5588199
CTCGCTC13650.029.5725523
TCTCGCT14050.029.2535722
GTCCTAT6250.027.707581
TTGCTCT30200.026.4634
GTATTAT8000.026.1562441
GTCTTAA8650.025.8590761
CGCTCTG15300.025.4112115
CTGTTGC26800.024.984539
GTATAAT7550.024.8481311
GTACTAA4950.024.780561
GTCTCAC28000.024.738911
TTACTCT12150.024.2957674
CTTGCTC30300.024.2655963
Produced by FastQC (version 0.11.5)