FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107705
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC18600.035.620641
TCTGTCG12650.030.734938
TCGCTCT11750.030.0252914
GTCTCGC13100.029.6858371
TCTCGCT14650.027.03052
GTGTTAC7850.026.6045061
GGGTTTC35550.026.1322571
CTCGCTC14350.026.0903843
GTCTCAC26100.025.3848231
GTTTCAC31200.025.384413
GTACTTA4151.8189894E-1224.288966
GTCTTAC8450.023.8631731
TTATACT7550.023.8408684
TATACTT6350.023.8108335
TTGCTCT27150.023.602024
GTACTAA4950.023.2777841
TAGTACT4950.023.2725414
GTATTAG7750.023.2308521
TATACTG7750.023.225625
GTGTTAG16200.022.671591
Produced by FastQC (version 0.11.5)