FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885931
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12150.047.9982348
TCGCTCT12900.041.8589254
GTCTCGC14500.039.81581
GTCTTGC23150.038.96661
TCTCGCT14800.037.517232
CTCGCTC15700.037.1921733
CTGTCGC16750.036.535979
CGCTCTG16050.032.2978745
GTTTCAC34050.031.3336333
GTGTTAC10950.030.9754241
GGGTTTC37050.030.7753051
TTGCTCT31250.029.2597244
CTTGCTC30600.027.7988433
TTCACCG7850.027.5149125
TCTGTCA28950.027.3565068
GTCTCAC27000.026.9954831
ACCGTGT9200.026.6077188
GCGTTAC1907.4229483E-626.5875281
GGTTTCA36950.026.3463882
GTGTAAA13150.025.793221
Produced by FastQC (version 0.11.5)