FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2818499
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.052.8219388
GTCTCGC11250.037.198981
CTGTCGC14700.036.7331779
TCGCTCT11750.035.538964
GTCTTGC23950.032.2354551
TCTCGCT13000.031.6122322
TATACTT6350.029.4791015
CTCGCTC13300.029.2656233
TTATACT7350.027.427444
CGCTCTG14700.027.427445
TTCACCG7700.027.1157635
GTACTAC4750.025.8232461
GTCTTAC8950.025.7977891
GTAATAC6750.025.6284963
TTGCTCT30550.025.2166374
ACCGTGT9200.025.0424428
TAATACT7500.024.958974
GGGTTTC40950.024.6677551
TCACCGT8800.024.5444416
TACACTG15350.023.9208375
Produced by FastQC (version 0.11.5)