FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640341
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8850.031.7274078
GTCTCGC9850.027.841851
GTCTTGC18600.027.5483881
GTCTCAC21000.025.7746491
TAACACT8950.025.7418774
GTAACAC7300.025.6796593
GGTATAC5100.025.4714161
TCGCTCT11100.024.6475564
GTACTAT4400.024.6030731
GTGTAAA12500.023.0940841
TTACACT8800.022.9080774
GGGTTTC31200.022.6684721
GTGTTAC8100.022.2743871
CTGTCGC13300.022.1945069
TCTCGCT12250.021.7843422
TCACTCT26150.021.7504714
GTGTAAC7050.021.4971521
TTGCTCT23150.021.4590934
TATTACG1700.003188889721.212892
GTCTAGC8900.021.0830821
Produced by FastQC (version 0.11.5)