FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640341
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10850.031.852118
GTCTTAC6750.030.9376051
GTCTCGC9950.026.7775651
GTCTTGC18750.026.1156041
GTATAAG6200.025.5519141
TCGCTCT10450.023.4255224
GTCGTAA1851.9179321E-423.3545781
GTCTAAG7000.022.6316971
GTATTAG6050.022.614691
GTAACCG1952.7419644E-422.1535474
TAATACT6250.021.8877074
CGCTCTG12500.021.8877075
GTCTCAC21050.021.551671
GTTTCAC27300.021.362353
CTCGCTC11500.021.286673
GTGTTAC7150.021.1497731
TACACTT7550.020.979855
GTATAGG6550.020.888381
GTCTTAG8000.020.702861
TCTGTTG19500.020.6766458
Produced by FastQC (version 0.11.5)