FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484256
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.042.3522728
GTCTCGC10400.038.851511
TCGCTCT10900.037.650784
TATACTG5500.035.34495
GTCTTAC8350.034.5642171
TAACCCG2206.712071E-1032.7267575
GTCTTGC19900.032.631921
CTGTCGC13950.032.5156149
GTATAAT5450.031.7737081
TCTCGCT12650.030.7774372
CTCGCTC13150.030.1493973
CGCTCTG13500.029.3328695
GTTTCAC34950.028.8750363
GTGTAAC6300.028.6320651
TTATACT5100.028.2348484
TTACTCT12550.028.111114
GGGTTTC37300.027.4683571
CTCGATC8150.027.386078
CCGTTAT1607.059088E-527.05731
TCTCGAT8550.026.1048537
Produced by FastQC (version 0.11.5)