FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484256
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9850.036.5487068
GTCTTGC19200.034.5060121
GTCTCGC10550.032.7640041
TAATACT6400.032.625434
GTCCTAT6400.031.5054911
TCGCTCT11100.027.2436074
GTGTTAC8300.026.8962551
GTGTAAA12050.026.8926541
GGGTTTC33550.025.9717391
GTTTCAC30050.025.6376113
TACACTG13300.025.4439475
GTATTAT6550.025.286851
GTTCTAC6850.025.2306771
CTGTCGC15800.025.0636239
CTCGCTC12550.024.6696513
GTCCTAA7450.024.1652871
TCTCGCT13150.024.091092
GTCTAAC4251.8189894E-1223.721781
CCGTGTT7650.023.5297249
GTATTAG8000.023.404081
Produced by FastQC (version 0.11.5)