FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499059
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5350.052.483618
GTCTCGC6850.044.25471
CTGTCGC6400.042.748079
TCGCTCT6800.042.351034
GTCTTGC11650.038.411961
ACTTTCG1201.0034939E-535.9983758
TCTCGCT7950.035.379342
GTATTAG5050.034.3020861
TTCACCG2800.033.4270635
CTCGCTC8000.033.3452073
GTTTCAC18450.032.4342163
CGCTCTG7800.032.3062325
GTACTAT3500.030.9331321
TTACACT5600.030.855754
GGGTTTC21100.028.7340931
TTACTCT7050.028.5944524
TCTCGAT5400.027.9987347
GTGTTAC5250.027.4961171
TAATACT3453.6379788E-1227.129214
GGTTTCA20350.026.9340362
Produced by FastQC (version 0.11.5)