FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499059
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6000.044.3994378
GTCTCGC7200.042.0092741
TCGCTCT7150.040.2792054
GTCTTGC12250.038.80041
GTGTTAC6300.037.722611
CGCTCTG7400.035.9995465
CTCGCTC8050.034.8815463
TCTCGCT8400.034.285282
GTATTAC2551.8189894E-1233.8898351
GGCGTAC1102.5344628E-432.7344971
TACCCCG1551.5472033E-632.5157135
TTATACT4450.032.359144
CTGTCGC9250.031.9131059
TTACACT6500.031.014994
TACCTCG1255.3685164E-428.7996337
GTATTAG4350.028.1441441
AATCCGA1804.9625833E-627.9996436
GTAACGC1555.7683632E-527.8706113
GTACGGT1306.752708E-427.6919563
TATACTT3403.6379788E-1227.5290625
Produced by FastQC (version 0.11.5)